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KMID : 0613820230330010001
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Journal of Life Science
2023 Volume.33 No. 1 p.1 ~ p.7
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Glutamate-rich 4 Binds to Kinesin Superfamily Protein 5A
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Pyo Se-Young
Jeong Young-Joo
Park Sung-Woo
Seo Mi-Kyoung
Lee Won-Hee
Urm Sang-Hwa
Kim Sang-Jin
Kim Moo-Seong
Lee Jung-Goo
Seog Dae-Hyun
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Abstract
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Intracellular cargo transport is mediated by molecular motor proteins, such as kinesin and cytoplasmic dynein. Kinesins make up a large subfamily of molecular motors. Kinesin-1 is a plus-end-directed molecular motor protein that moves various cargoes, such as organelles, protein complexes, and mRNAs, along a microtubule track. It consists of the kinesin superfamily protein (KIF) 5A, 5B, and 5C (also called kinesin heavy chains) and kinesin light chains (KLCs). Kinesin-1 interacts with many different binding proteins through its carboxyl (C)-terminal region of KIF5s and KLCs, but their binding proteins have not yet been fully identified. In this study, a yeast two-hybrid assay was used to identify the proteins that interact with the KIF5A specific C-terminal region. The assay revealed an interaction between KIF5A and glutamate-rich 4 (ERICH4). ERICH4 bound to the KIF5A specific the C-terminal region but did not interact with the C-terminal region of KIF5B or KIF3A (a motor protein of kinesin-2). In addition, KIF5A did not interact with another isoform, ERICH1. Glutathione
S-transferase (GST) pull-downs showed that KIF5A interacts with GST-ERICH4 and GST-ERICH4- amino (N)-terminal but not with GST-ERICH4-C or GST alone. When co-expressed in HEK-293T cells, ERICH4 co-localized with KIF5A and co-immunoprecipitated with KIF5A and KLC but not KIF3B. Together, our findings suggest that ERICH4 is capable of binding to KIF5A and that it may serve as an adaptor protein that links kinesin-1 with cargo.
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KEYWORD
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Adaptor protein, binding protein, glutamate-rich, kinesin-1, microtubule
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